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apoptotic morphology analysis apoptotic morphological changes  (Beyotime)


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    Beyotime apoptotic morphology analysis apoptotic morphological changes
    Apoptotic Morphology Analysis Apoptotic Morphological Changes, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apoptotic+morphological+changes/Hoechst+33258+Staining+Solution/pm27804109-78-0-14
    Average 99 stars, based on 491 article reviews
    apoptotic morphology analysis apoptotic morphological changes - by Bioz Stars, 2026-09
    99/100 stars

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    Staining:

    Article Title: Daphnoretin-induced apoptosis in HeLa cells: a possible mitochondria-dependent pathway
    Article Snippet: Fluorescent images were visualized by a Zeiss inverted fluorescence microscope. .. Daphnoretin-induced apoptotic morphological changes were analyzed by Hoechst staining (Hoechst 33258 kit; Beyotime). ..

    Article Title: Daphnoretin-induced apoptosis in HeLa cells: a possible mitochondria-dependent pathway
    Article Snippet: Fluorescent images were visualized by a Zeiss inverted fluorescence microscope. .. Apoptotic morphology analysis Daphnoretin-induced apoptotic morphological changes were analyzed by Hoechst staining (Hoechst 33258 kit; Beyotime). ..



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    characteristic apoptotic morphological changes  (Beyotime)
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    Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and <t>apoptotic</t> morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.
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    apoptotic morphological changes  (Beyotime)
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    Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and <t>apoptotic</t> morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.
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    Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and apoptotic morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Arenobufagin Induces Apoptotic Cell Death in Human Non-Small-Cell Lung Cancer Cells via the Noxa-Related Pathway

    doi: 10.3390/molecules22091525

    Figure Lengend Snippet: Arenobufagin induces caspase-dependent apoptosis in non-small-cell lung cancer (NSCLC) cells. ( A ) A549 and NCI-H460 cells were treated with the indicated concentrations of arenobufagin for 24 h, and apoptotic morphological changes were evaluated by Hoechst 33258 staining; ( B , C ) A549 and NCI-H460 cells were treated with arenobufagin at the indicated concentrations for 24 h, and protein extracts were subjected to Western blot assay with indicated antibodies; ( D , E ) A549 cells were pre-treated with the caspase inhibitor Z-VAD (40 μΜ) for 1 h, followed by incubation with arenobufagin (Are, 25 nM) for 24 h; ( D ) poly (ADP-ribose) polymerase (PARP) cleavage was analyzed by Western blotting; ( E ) The cell viability was detected via trypan blue exclusion assay. ** p < 0.01, arenobufagin-treated group versus arenobufagin and Z-VAD combination group.

    Article Snippet: Characteristic apoptotic morphological changes were assessed by Hoechst 33258 staining, using the Hoechst staining kit (Beyotime, Haimen, China).

    Techniques: Staining, Western Blot, Incubation, Trypan Blue Exclusion Assay